Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134828_4ji2 GATGCAGTCCAGACTTGTGTTTAAGTTTACAG(+AGGACA)GTACCTCTTGGACTCCTGAATTGATCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240613134826_ftkv GAACTAACTCACAGGCTCTTGTCAAAGTCTGG(+AGGACA)GTACCAAATTTAGAAGTCTTTTTGATGTACC NGG 10 8 17 8 24 0 100 5 10
    240613134825_sz2s AAGAGGGTACATCAAAAAGACTTCTAAATTTG(+AGGACA)GTACCCAGACTTTGACAAGAGCCTGTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613134824_o8qw TCTACTTAATTAGCGACCAGCAAACACTAATG(+AGGACA)GATCCATGGGGTAGGGTTTTTGGTTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613134821_yjbu AACAACAACAAAACCAAAAACCCTACCCCATG(+AGGACA)GATCCATTAGTGTTTGCTGGTCGCTAATTAA NGG 10 8 17 8 24 0 100 5 10
    240613134820_bszc GTCATCCTTGTGATGGCATGCTCCTTTGCTGG(+AGGACA)GTACCATGTAGGGCCATTTTTTTCCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613134819_0e3v AAACTGCAGGGGGAAAAAAATGGCCCTACATG(+AGGACA)GTACCCAGCAAAGGAGCATGCCATCACAAGG NGG 10 8 17 8 24 0 100 5 10
    240613134817_qxjt AAGTGCACGCATGTGCAGGAATGTCGCAGTGG(+AGGACA)GATCCGGAGATCGCGGAGACCGTGGAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240613134815_xrq4 AGGAACGGGCTCCACGGTCTCCGCGATCTCCG(+AGGACA)GATCCCACTGCGACATTCCTGCACATGCGTG NGG 10 8 17 8 24 0 100 5 10
    240613134814_hudr GCTGTCTTTGGGCACATCAGAAGAGGGCATTG(+AGGACA)GATCCCATTAATGGATGGTTGTGAGCCATCT NGG 10 8 17 8 24 0 100 5 10