Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134601_g0mq AGCGAAAATAAGCACGCTATTGACATCTGGTG(+AGGACA)GATCCTGTCCCCAGTGGAGGAGGAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613134600_mr17 CGGCACGGCCCGCCCGGCCCCTCCACCAGGAG(+AGGACA)GATCCGAAAGGTTACCTGTACAGTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613134558_u7ea AGGCTACAGGAAACTGTACAGGTAACCTTTCG(+AGGACA)GATCCTCCTGGTGGAGGGGCCGGGCGGGCCG NGG 10 8 17 8 24 0 100 5 10
    240613134557_aj7w TGGAGTCCACTGCTGGCCAGCCCCAGCACTGG(+AGGACA)GTACCCACAGGACATGCCCCCACACACCTCA NGG 10 8 17 8 24 0 100 5 10
    240613134555_20nj TGTTGTGAGGTGTGTGGGGGCATGTCCTGTGG(+AGGACA)GTACCCAGTGCTGGGGCTGGCCAGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613134553_fvrs TGTAGTCCCTCTTATCCGGCTGGATTAACCTG(+AGGACA)GTACCCCAACATTATCCAGATTTCCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240613134551_d3q1 GTTTTGGAGTAGGAAATCTGGATAATGTTGGG(+AGGACA)GTACCAGGTTAATCCAGCCGGATAAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613134549_cpf1 CATCTCTGGAACTCGGATTCCCATCGTATTGG(+AGGACA)GTACCACTGCTTTTAAAGCACTAAGTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613134548_1ukq TATGTTTAACACTTAGTGCTTTAAAAGCAGTG(+AGGACA)GTACCCAATACGATGGGAATCCGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613134547_bm8z GAGCATCCTCTGTCCTGCCTCGTGCCCAGATG(+AGGACA)GTACCTCTGGAGAAGGCATGCGGCTGGCCTC NGG 10 8 17 8 24 0 100 5 10