Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134527_8mwb ACCCCACTCCCCCATCTGGATGTGACCAGCTG(+AGGACA)GATCCAACCACAGTGTGCCCCAGACCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240613134525_f986 TTATGATCCCGGTGCTGAGGAGGTGGTGGCGG(+AGGACA)GATCCCTGGAGCTCACTGGCAAGCCTCATCA NGG 10 8 17 8 24 0 100 5 10
    240613134523_e8qi CTCCCTGATGAGGCTTGCCAGTGAGCTCCAGG(+AGGACA)GATCCCGCCACCACCTCCTCAGCACCGGGAT NGG 10 8 17 8 24 0 100 5 10
    240613134522_xuit TGTGGCCCTCCTGGAGCAACCTGGCAGCTACG(+AGGACA)GATCCCAGAACCCCCTGGGCTTCAGCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613134520_ijc9 CTCTGGGGGAGCTGAAGCCCAGGGGGTTCTGG(+AGGACA)GATCCGTAGCTGCCAGGTTGCTCCAGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613134518_xte7 CAGCACGAGGCTCGCCGTGAAGGGTGGCCTCG(+AGGACA)GATCCTGGGCACGGCACGGTGACACTGCAAC NGG 10 8 17 8 24 0 100 5 10
    240613134516_695w CATCTGTTGCAGTGTCACCGTGCCGTGCCCAG(+AGGACA)GATCCGAGGCCACCCTTCACGGCGAGCCTCG NGG 10 8 17 8 24 0 100 5 10
    240613134513_b6co TAGGGGCGCAGGGCGCGTGGAACCTAGCTGGG(+AGGACA)GATCCAGCTTGCCTGGGTTTCTGGGGTGGTA NGG 10 8 17 8 24 0 100 5 10
    240613134512_wyrk CTGTTTACCACCCCAGAAACCCAGGCAAGCTG(+AGGACA)GATCCCCAGCTAGGTTCCACGCGCCCTGCGC NGG 10 8 17 8 24 0 100 5 10
    240613134510_ob7r CCGAGTCCAGGTGCCCATGGAAGAAAGCATTG(+AGGACA)GATCCCCCTGGAGTTGAAGTCACCTAATGTA NGG 10 8 17 8 24 0 100 5 10