Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134419_ji4z AAAAAAATCACACTGTTATTTTTGGCCAGTGG(+AGGACA)GTACCAGGCCCCATGGACCTGTCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613134416_pxdr CAAAGCAGCAGAGAATAGAGGACGGAGCCCAG(+AGGACA)GTACCTCCTCTTCCTCCTCCTCACCGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240613134415_q09q ATGGAGGCGACGGTGAGGAGGAGGAAGAGGAG(+AGGACA)GTACCTGGGCTCCGTCCTCTATTCTCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613134413_1ub8 TGGGAAATAGACCCTGCAGTTACCTAGAATGG(+AGGACA)GTACCAGAGAGATGATGCTGGAAGCGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240613134412_fifx ATACTGTCGGCGCTTCCAGCATCATCTCTCTG(+AGGACA)GTACCCATTCTAGGTAACTGCAGGGTCTATT NGG 10 8 17 8 24 0 100 5 10
    240613134411_64u2 AAAACCAATTTACTGAAAAACTGCACAAGAAG(+AGGACA)GTACCGTGAATGTAATTTCCTAGCAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240613134410_7hlj GCAGAGTGGCCTGCTAGGAAATTACATTCACG(+AGGACA)GTACCTTCTTGTGCAGTTTTTCAGTAAATTG NGG 10 8 17 8 24 0 100 5 10
    240613134407_y9fr TTTTCTTTGGAGTCTGGATTTGGTGCCCGTGG(+AGGACA)GATCCTGGAGGGCCCCACCCCCATCCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613134405_31zj AGAGACTTCAGGATGGGGGTGGGGCCCTCCAG(+AGGACA)GATCCCACGGGCACCAAATCCAGACTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613134404_sd7k CAATCCCAGCATTCAAGAGGTAGAGGCAGGAG(+AGGACA)GATCCGGAGTTCAAAGTCATCCTTGGCTGGA NGG 10 8 17 8 24 0 100 5 10