Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134450_9so3 AGAAGTGGCAGCTGCCGCTGTGAGCCACGCAG(+AGGACA)GATCCCGTGTCCTGCAGACGAGAGCGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613134449_ouz2 CCAGTTTTTCTCAGATTTAGGGAGAATACATG(+AGGACA)GTACCTTTTACCTACCCATACTAGAAACTAA NGG 10 8 17 8 24 0 100 5 10
    240613134448_8hp5 TGTAGTTAGTTTCTAGTATGGGTAGGTAAAAG(+AGGACA)GTACCATGTATTCTCCCTAAATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613134445_fv4p AAGTGGGGAGGAATGGTACTATTGGCATGGGG(+AGGACA)GTACCTGGTATTGAAAATGGACACATAAACT NGG 10 8 17 8 24 0 100 5 10
    240613134443_1y9p CAGCTAGTTTATGTGTCCATTTTCAATACCAG(+AGGACA)GTACCCCCATGCCAATAGTACCATTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613134442_rcfp GGGGATCTGATGTCCTCTTCTGGCCTCTGTTG(+AGGACA)GTACCTGCACACACATGGTGTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240613134441_w3x8 TATTTGTGTATGTGTACACCATGTGTGTGCAG(+AGGACA)GTACCAACAGAGGCCAGAAGAGGACATCAGA NGG 10 8 17 8 24 0 100 5 10
    240613134440_iozk GATTCTAGTCTGACTTGGAACAGAGGTCACAG(+AGGACA)GTACCAACCTGATGAGACCTGCTGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240613134438_kcn0 GAGACACAATAGCAGCAGGTCTCATCAGGTTG(+AGGACA)GTACCTGTGACCTCTGTTCCAAGTCAGACTA NGG 10 8 17 8 24 0 100 5 10
    240613134437_jmxa AATACATTATAGCATGTCCTGTAGGTCTGAAG(+AGGACA)GTACCATAGTTCAGGTACTCACATGATTTAC NGG 10 8 17 8 24 0 100 5 10