Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134347_bttw GTTTCCCCCAACCCCACCTCATGTGTATTAGG(+AGGACA)GATCCAAGTAGGTGCCAGCAGAGATGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613134345_jrhk ATATAATGTGGAGGCAGCAGAGGGGTAAGTGG(+AGGACA)GTACCTAGCAGGCCCATGCCAAGGTGTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613134344_rhql CTCAGGAGGACACCTTGGCATGGGCCTGCTAG(+AGGACA)GTACCCACTTACCCCTCTGCTGCCTCCACAT NGG 10 8 17 8 24 0 100 5 10
    240613134343_sr04 TGTGACCAATTATATGGTCAGTTTTGGAGAAG(+AGGACA)GTACCATAAGGTGATGAGAAGGAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240613134341_lb47 AAAAGAATATACCTCCTTCTCATCACCTTATG(+AGGACA)GTACCTTCTCCAAAACTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240613134340_uem3 CCCAAGCACCAGGAGAACGCAAACTAGTCTTG(+AGGACA)GTACCAATGCTGTGAACTATAACTTGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613134339_2e8x CTGCCCCAGCCAAGTTATAGTTCACAGCATTG(+AGGACA)GTACCAAGACTAGTTTGCGTTCTCCTGGTGC NGG 10 8 17 8 24 0 100 5 10
    240613134337_fll2 TCCCTCGATTACTCCCAGTTCAGGGAAAAGGG(+AGGACA)GTACCAACGTGCAGACTCCTGACCAGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613134335_jn38 TACCCGCCAGCTGGTCAGGAGTCTGCACGTTG(+AGGACA)GTACCCCTTTTCCCTGAACTGGGAGTAATCG NGG 10 8 17 8 24 0 100 5 10
    240613134333_i47p CTGCCTCCAGAACACTGCAGGATTCTCCACCG(+AGGACA)GTACCTGCTGGTTTCCCATAGGCCCAGGCTG NGG 10 8 17 8 24 0 100 5 10