Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134627_h0jl TTCCTTGGGTAACACCACACATACAGGTATGG(+AGGACA)GATCCCACAACAGACAAATGTACAGACACCA NGG 10 8 17 8 24 0 100 5 10
    240613134625_qwl8 TTTGGTGGTGTCTGTACATTTGTCTGTTGTGG(+AGGACA)GATCCCATACCTGTATGTGTGGTGTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240613134624_httj GGATGGCGGGCAAGGCCTATTAGTTCATCACG(+AGGACA)GATCCGGTTCAGGTGCCCTAGAGCCCCGCTG NGG 10 8 17 8 24 0 100 5 10
    240613134622_emq9 CTAAGCAGCGGGGCTCTAGGGCACCTGAACCG(+AGGACA)GATCCGTGATGAACTAATAGGCCTTGCCCGC NGG 10 8 17 8 24 0 100 5 10
    240613134620_euuz CCCTGACAAAACTCATTCTACCCAGTACCCAG(+AGGACA)GATCCTGGGCACACGCACATCTATATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613134617_aqnk AACCATTTCCATATAGATGTGCGTGTGCCCAG(+AGGACA)GATCCTGGGTACTGGGTAGAATGAGTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613134616_qjla GAGGATTGACTCCTCAGAGAAGGAGCCTATGG(+AGGACA)GTACCTGGGGCCACGTACAGCAGGGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240613134613_ge15 CTGCCGCTGAGCCCTGCTGTACGTGGCCCCAG(+AGGACA)GTACCCATAGGCTCCTTCTCTGAGGAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613134612_0217 CTTGGAAAGATGGGCCACATGCTGCATGACAG(+AGGACA)GATCCTCCAAGTACCTTAACTGTTAGAGTAT NGG 10 8 17 8 24 0 100 5 10
    240613134610_7gwb CACGGATACTCTAACAGTTAAGGTACTTGGAG(+AGGACA)GATCCTGTCATGCAGCATGTGGCCCATCTTT NGG 10 8 17 8 24 0 100 5 10