Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134732_kyrb TGTGCTCACTCCCTGGTGAACAGAGGAGCAGG(+AGGACA)GATCCCAGAAAATAAAACGTGGAGGTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613134730_x0s8 TGGAAAAGAGACCTCCACGTTTTATTTTCTGG(+AGGACA)GATCCCTGCTCCTCTGTTCACCAGGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613134729_8yk9 CAGTGAGCAGCATATTTTATATACTGGTTGAG(+AGGACA)GATCCCTAGTGAACATTATGACATCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240613134728_08uv TTCATAGTTCAGATGTCATAATGTTCACTAGG(+AGGACA)GATCCTCAACCAGTATATAAAATATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613134727_2dmk GGGCTTGACTCTGATTGATGGCCTCTGGAGAG(+AGGACA)GATCCTGCAGCTACCCAGATTCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613134725_q4qv TTGGAGCCAACCTTGAATCTGGGTAGCTGCAG(+AGGACA)GATCCTCTCCAGAGGCCATCAATCAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613134724_p96r ACAGGGTAATTCATTAGCAAGACATTTCATTG(+AGGACA)GTACCGGGTTCAGGTTGTCTGGGGAATAATG NGG 10 8 17 8 24 0 100 5 10
    240613134722_3017 CCTCCCATTATTCCCCAGACAACCTGAACCCG(+AGGACA)GTACCAATGAAATGTCTTGCTAATGAATTAC NGG 10 8 17 8 24 0 100 5 10
    240613134719_w5yy CCCCCGGGTCCAGCTCTGCTCCTGTGCCTGGG(+AGGACA)GATCCGGAGGCTCCTGGCTGTCTTGAAATGG NGG 10 8 17 8 24 0 100 5 10
    240613134717_qihm AAAGTCCATTTCAAGACAGCCAGGAGCCTCCG(+AGGACA)GATCCCCAGGCACAGGAGCAGAGCTGGACCC NGG 10 8 17 8 24 0 100 5 10