Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134954_m96g CATAATGGCAAACAGGCTGCAGGACCAGACTG(+AGGACA)GATCCTCAGCCCTGAAGTGTGCCCTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613134952_uvq8 ACCACACATATGTAATTCAGAGAACACTTCCG(+AGGACA)GATCCTTCACCCTCCCTCTAACACATCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613134951_siin AGGGTAGAGGATGTGTTAGAGGGAGGGTGAAG(+AGGACA)GATCCGGAAGTGTTCTCTGAATTACATATGT NGG 10 8 17 8 24 0 100 5 10
    240613134949_rhtl CACTTTCCTCCCCCCAAAACCTGGCCCCAAGG(+AGGACA)GTACCCCCAAAACAAAAAAAAAGAAAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613134947_zkds CTTTTTTTTCTTTCTTTTTTTTTGTTTTGGGG(+AGGACA)GTACCCTTGGGGCCAGGTTTTGGGGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613134946_91fh GTGCTTCTTTTTCACTACAATCAGGATTCATG(+AGGACA)GTACCCTGGAAAGTTATCTCAGGACATCAAG NGG 10 8 17 8 24 0 100 5 10
    240613134944_z13r ATTAGCTTGATGTCCTGAGATAACTTTCCAGG(+AGGACA)GTACCATGAATCCTGATTGTAGTGAAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613134942_8ycw ACCAACATATTTTCTCATGCCTGGTCTCTCGG(+AGGACA)GTACCACTTTTGACATACTTTAGTACCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613134941_htlb TATCAAAAAGGTACTAAAGTATGTCAAAAGTG(+AGGACA)GTACCCGAGAGACCAGGCATGAGAAAATATG NGG 10 8 17 8 24 0 100 5 10
    240613134939_m31c CGTCATCCTAAACTCACCATCCTTAGAAACGG(+AGGACA)GATCCTCTCTAGAATATTGTCGCCGGTGACC NGG 10 8 17 8 24 0 100 5 10