Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135052_w1i9 TGTGCCTTGGGCATTAGTGGGGCACTCCAGGG(+AGGACA)GTACCTTTAGGAAGTGTAGGGATTACACATC NGG 10 8 17 8 24 0 100 5 10
    240613135051_1nan GAAGGAAGAGGGTTAAAACTTGTGATAATAAG(+AGGACA)GATCCTTCAAAACTCTGGTGCCGTCCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240613135050_6wvh CAGGTACTGAGGACGGCACCAGAGTTTTGAAG(+AGGACA)GATCCTTATTATCACAAGTTTTAACCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613135049_ppjb TCACTTTCCTCCTCTGCAATTAAGAAATTGTG(+AGGACA)GTACCCTCTTCAGAAACTACACCTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613135047_il8x CCCACGAAAAAAAGGTGTAGTTTCTGAAGAGG(+AGGACA)GTACCACAATTTCTTAATTGCAGAGGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613135046_fqh5 GAGGTCAACTACGAAGTGGCTGTTTCGCATTG(+AGGACA)GTACCCAGCTGTGCCTCCACTATTTGCACAC NGG 10 8 17 8 24 0 100 5 10
    240613135045_xsjq AAGATGTGTGCAAATAGTGGAGGCACAGCTGG(+AGGACA)GTACCAATGCGAAACAGCCACTTCGTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613135043_7l4j TCACTTTCCTCCTCTGCAATTAAGAAATTGTG(+AGGACA)GTACCCTCTTCAGAAACTACACCTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613135042_vb5y CCCACGAAAAAAAGGTGTAGTTTCTGAAGAGG(+AGGACA)GTACCACAATTTCTTAATTGCAGAGGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613135040_bd4l CTGGGACCTCCCATGTGTCTTTATTCCCAATG(+AGGACA)GTACCCAACTTGTCTCCTTCTACCTTGTTCT NGG 10 8 17 8 24 0 100 5 10