Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135135_pizf AAACATCCACTTGCCAAATTTTATTTCTTTGG(+AGGACA)GTACCTTTAGGGTTTCTTCCTGCAGGTAATA NGG 10 8 17 8 24 0 100 5 10
    240613135134_id82 GGTCTTGAACTCAGTATGTAGTCTACATTTTG(+AGGACA)GATCCTCCTGCCCCCACCTCCTAAGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240613135132_epby TAATGTCAACACTTAGGAGGTGGGGGCAGGAG(+AGGACA)GATCCAAAATGTAGACTACATACTGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240613135131_m3st CATGAATGTTTTGGACATCAAGCATCCTAATG(+AGGACA)GTACCTCCTTTGTACAATGCCAAAATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613135129_8k5g ACCGAGGCTCATTTTGGCATTGTACAAAGGAG(+AGGACA)GTACCATTAGGATGCTTGATGTCCAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240613135128_9ypb CTGGAGTTGGTCATTTTTGTACACCCACAGTG(+AGGACA)GTACCTTTTACAGTCAGGTTTGGATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240613135127_n37l AGCTGCAAAGTATCCAAACCTGACTGTAAAAG(+AGGACA)GTACCACTGTGGGTGTACAAAAATGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240613135125_dx6k CCCCCAGTTCTCTGGCTGAGGCAACATTCCTG(+AGGACA)GTACCAGTGCCTATCACTTTGTTTCAGGACT NGG 10 8 17 8 24 0 100 5 10
    240613135124_xv7u TAGGCAGTCCTGAAACAAAGTGATAGGCACTG(+AGGACA)GTACCAGGAATGTTGCCTCAGCCAGAGAACT NGG 10 8 17 8 24 0 100 5 10
    240613135122_pqmf GTGTGTGTGTGTGTGTGTGTGTGTACTCTAGG(+AGGACA)GTACCTCAGTATGTCCTGAAAGTTCAACAGA NGG 10 8 17 8 24 0 100 5 10