Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135149_i8kr AGTTGGGACACACCCCTGTATGATCTTACTTG(+AGGACA)GATCCTAAATTCTAAAGGAGAAAATAATTGA NGG 10 8 17 8 24 0 100 5 10
    240613135148_69rp ATTTATACTTTTTAAATAAATGGTACTTGCTG(+AGGACA)GATCCACACTAACATCTTTGCTAACAATCCC NGG 10 8 17 8 24 0 100 5 10
    240613135147_92rd ACAATGGGATTGTTAGCAAAGATGTTAGTGTG(+AGGACA)GATCCAGCAAGTACCATTTATTTAAAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613135145_wmuc TGAAATCAGCTCAGGTCTGAAATCGGTCTGTG(+AGGACA)GTACCTTGGGACCCTCAGGTCAGCAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613135143_mmmh CAGCAGCAGCCTGCTGACCTGAGGGTCCCAAG(+AGGACA)GTACCACAGACCGATTTCAGACCTGAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613135141_pcbn CCAGTATTTAACCACTCAATGCCAACTAAAAG(+AGGACA)GTACCAGTGAAAAGTTTTGCCACAAAGAATG NGG 10 8 17 8 24 0 100 5 10
    240613135140_gaqt TTCAACATTCTTTGTGGCAAAACTTTTCACTG(+AGGACA)GTACCTTTTAGTTGGCATTGAGTGGTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240613135139_08au AGTGAGCAGAAATAGACCCAGCAGAAATTGTG(+AGGACA)GTACCTCTTATTAATGCTGAGACTGCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613135138_r3wx TTTTAATGAGGCAGTCTCAGCATTAATAAGAG(+AGGACA)GTACCACAATTTCTGCTGGGTCTATTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613135136_gjn7 TACTCTATTACCTGCAGGAAGAAACCCTAAAG(+AGGACA)GTACCCAAAGAAATAAAATTTGGCAAGTGGA NGG 10 8 17 8 24 0 100 5 10