Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135121_o564 AAAACTCTGTTGAACTTTCAGGACATACTGAG(+AGGACA)GTACCCTAGAGTACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240613135120_bv8c GAAAAGCACCATGGGAAATCCCTTGGCTTCTG(+AGGACA)GTACCAGCATGGTAATTGCAAGGGAAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240613135119_1axl TCTGAATTTCTTCCCTTGCAATTACCATGCTG(+AGGACA)GTACCAGAAGCCAAGGGATTTCCCATGGTGC NGG 10 8 17 8 24 0 100 5 10
    240613135117_2zon AGAACTTGGGTCTGCTTTCTACAGTCATTATG(+AGGACA)GTACCAAAACCATATGATTCTTTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240613135116_9hg1 GGATAAACTGTACAAAGAATCATATGGTTTTG(+AGGACA)GTACCATAATGACTGTAGAAAGCAGACCCAA NGG 10 8 17 8 24 0 100 5 10
    240613135115_78wc AGCTTGTTTCTGAGAAAACCAAGTTCTAAGTG(+AGGACA)GTACCCAAAGGTGTTGCCAGTCCTTCCTATG NGG 10 8 17 8 24 0 100 5 10
    240613135113_nxts GGATGCATAGGAAGGACTGGCAACACCTTTGG(+AGGACA)GTACCACTTAGAACTTGGTTTTCTCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613135112_dsq9 CTCCATGGTTAAGAACACTAGTTTTTGCTGAG(+AGGACA)GATCCCAGTTCAATTCCCAGTACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240613135111_ko2p AGCCACCATGTGGGTACTGGGAATTGAACTGG(+AGGACA)GATCCTCAGCAAAAACTAGTGTTCTTAACCA NGG 10 8 17 8 24 0 100 5 10
    240613135109_cv54 CTGGATGCCGGTGATGCTGGAGTAGACGGCTG(+AGGACA)GATCCCACCACGCACATTTCACGTTATTTGA NGG 10 8 17 8 24 0 100 5 10