Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135107_f1gv ACTAATCAAATAACGTGAAATGTGCGTGGTGG(+AGGACA)GATCCAGCCGTCTACTCCAGCATCACCGGCA NGG 10 8 17 8 24 0 100 5 10
    240613135106_h1gv GATCACTCTGAAGGTCTGATTGTCCTCTCTTG(+AGGACA)GATCCCCTTGAGGAAGAGTAACCCTGTCACA NGG 10 8 17 8 24 0 100 5 10
    240613135104_24z4 TGCTGTGTGACAGGGTTACTCTTCCTCAAGGG(+AGGACA)GATCCAAGAGAGGACAATCAGACCTTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613135103_1r7f CATCAGTTTAGGGATGGCCAGGCCATAGTGAG(+AGGACA)GTACCTGACCAGATCTCAGAGTCAGGCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613135101_2lfn TGGGAGGGAGCCTGACTCTGAGATCTGGTCAG(+AGGACA)GTACCTCACTATGGCCTGGCCATCCCTAAAC NGG 10 8 17 8 24 0 100 5 10
    240613135100_p76u TCAAGCATCATGCTTACAATTGAGCAGCTGAG(+AGGACA)GTACCATCGCAGATGGAGGTAAAGGGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613135059_fd5k CAGGAGCTGCCCCTTTACCTCCATCTGCGATG(+AGGACA)GTACCTCAGCTGCTCAATTGTAAGCATGATG NGG 10 8 17 8 24 0 100 5 10
    240613135057_uupk TGTCAAGTTACCATAGCAACCAGCAACTCCAG(+AGGACA)GTACCACAAAAGACAATGAGCTGAGGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613135056_4xyj CTCTCTCACACCTCAGCTCATTGTCTTTTGTG(+AGGACA)GTACCTGGAGTTGCTGGTTGCTATGGTAACT NGG 10 8 17 8 24 0 100 5 10
    240613135055_7vhu TTCTGGATGTGTAATCCCTACACTTCCTAAAG(+AGGACA)GTACCCCTGGAGTGCCCCACTAATGCCCAAG NGG 10 8 17 8 24 0 100 5 10