Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132925_12in TCTTGTTTTATTGAATTACACAGCCCCTACTG(+AGGACA)GTACCTCTTGCTACTCTTAATTATAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613132923_g8yl GGAGATCTAGTGTACTCTTCTGACCTCCAAAG(+AGGACA)GTACCAGGTGAACATCCGGATGAATGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613132921_3bu6 ATTTTGCCTGCATTCATCCGGATGTTCACCTG(+AGGACA)GTACCTTTGGAGGTCAGAAGAGTACACTAGA NGG 10 8 17 8 24 0 100 5 10
    240613132920_lxxu TCTAAAATGTATCATACATTAACGCAGGGCAG(+AGGACA)GATCCATTAACATGGCTCCGCCTTTACAAGT NGG 10 8 17 8 24 0 100 5 10
    240613132918_p406 CTGCCACTTGTAAAGGCGGAGCCATGTTAATG(+AGGACA)GATCCTGCCCTGCGTTAATGTATGATACATT NGG 10 8 17 8 24 0 100 5 10
    240613132917_4f4l ACGAGTCATTGCTTCTGGTTTGTCCTAACAGG(+AGGACA)GTACCTGACAGGAAATTGCTAGCTCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613132915_fiob AGTTTCTGCCAGAGCTAGCAATTTCCTGTCAG(+AGGACA)GTACCCTGTTAGGACAAACCAGAAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240613132914_3m37 GTAGGGTGTTCCTCCCAGTTCAGGTAGTGTTG(+AGGACA)GATCCTGAGTTGTGGCTGGGATAACTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613132913_3jnr AATCCTGAAGAGTTATCCCAGCCACAACTCAG(+AGGACA)GATCCAACACTACCTGAACTGGGAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240613132912_aa1v TTACTCTCAAGGCTTCATGATCGACAATGAAG(+AGGACA)GATCCTGCAGGAACGGAGGTGGTGGTGGCAC NGG 10 8 17 8 24 0 100 5 10