Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133015_kydl GAACCCACTGTTGAGTGCTGACCATGAGAATG(+AGGACA)GATCCATGGGCCAAGCAGGTCACCTATGGAT NGG 10 8 17 8 24 0 100 5 10
    240613133014_3aca AAATAAAAAAGAGAAACTCTGGGAACCTCTGG(+AGGACA)GATCCGAGGCAGCCAATACAGTGGTGTAAGT NGG 10 8 17 8 24 0 100 5 10
    240613133012_hnm6 ATCCAACTTACACCACTGTATTGGCTGCCTCG(+AGGACA)GATCCCAGAGGTTCCCAGAGTTTCTCTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613133010_6nn8 TCCACTGTCTAAGTCTGTAACATAACAAACGG(+AGGACA)GATCCTGTCTTCTTCTTTTCTAATAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240613133009_mvhx TTACAAAATTCTATTAGAAAAGAAGAAGACAG(+AGGACA)GATCCCGTTTGTTATGTTACAGACTTAGACA NGG 10 8 17 8 24 0 100 5 10
    240613133006_ezx9 ACGGGCTAATCTGCTTGTCATGAAGCCCCAAG(+AGGACA)GATCCCTCTCTGCTTCTCCACTGCTGACATT NGG 10 8 17 8 24 0 100 5 10
    240613133004_luh8 GTTGCAATGTCAGCAGTGGAGAAGCAGAGAGG(+AGGACA)GATCCTTGGGGCTTCATGACAAGCAGATTAG NGG 10 8 17 8 24 0 100 5 10
    240613133003_pclc TAAAAAGTCTTGCCTCTGAAGACCTTTTAAAG(+AGGACA)GTACCATGAGTAGGAATTGGGAAGAATTGTT NGG 10 8 17 8 24 0 100 5 10
    240613133002_6pgj ATTGTAACAATTCTTCCCAATTCCTACTCATG(+AGGACA)GTACCTTTAAAAGGTCTTCAGAGGCAAGACT NGG 10 8 17 8 24 0 100 5 10
    240613133000_xnby TTTCTTAATGTTCTAATTATAATGAAAGTCGG(+AGGACA)GATCCCATGTCACACTTTGGGAAACCTTTGA NGG 10 8 17 8 24 0 100 5 10