Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133047_7mqm CACCCGTGTGCTTCCTTCCTTCTTGGAATCCG(+AGGACA)GTACCAGCTGCTGTTGAATTTTCTCATGAAG NGG 10 8 17 8 24 0 100 5 10
    240613133045_gyez AAGTCACAGACTTTAAAAGTAGGTATGGGCGG(+AGGACA)GATCCATCCAAGCCATCACTGGTCAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613133043_covt GATGTCTCTCCTGACCAGTGATGGCTTGGATG(+AGGACA)GATCCCGCCCATACCTACTTTTAAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613133042_l1zp GTCCCTGTGCTGATGGGGAGGAGGAGGGCAGG(+AGGACA)GTACCGGGAGCAGGTAGCAGAAGGAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613133040_2cy0 AAGGCAGAGGCTCCTTCTGCTACCTGCTCCCG(+AGGACA)GTACCCTGCCCTCCTCCTCCCCATCAGCACA NGG 10 8 17 8 24 0 100 5 10
    240613133038_hbo7 GAGCTGACCTTCCCAGGAACCTCACAGTTCCG(+AGGACA)GATCCTTTGTCTCAGTAGATACAGCTTAGCC NGG 10 8 17 8 24 0 100 5 10
    240613133037_b1cx CATGAGGCTAAGCTGTATCTACTGAGACAAAG(+AGGACA)GATCCGGAACTGTGAGGTTCCTGGGAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613133034_9s41 CTGCTAGGAAGCACACAAGTTTCATCACAGGG(+AGGACA)GTACCCAAGTGCCTCATACCATTTATGAATT NGG 10 8 17 8 24 0 100 5 10
    240613133033_d9h0 ATCTAAATTCATAAATGGTATGAGGCACTTGG(+AGGACA)GTACCCCTGTGATGAAACTTGTGTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613133031_k4r3 CTGCTAGGAAGCACACAAGTTTCATCACAGGG(+AGGACA)GTACCCAAGTGCCTCATACCATTTATGAATT NGG 10 8 17 8 24 0 100 5 10