Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133216_x8rf AGAGATCGCCTGCCTTGTGTCATGAGTGCTGG(+AGGACA)GATCCATAAGCCACCATGCTTGGCTGATGTT NGG 10 8 17 8 24 0 100 5 10
    240613133215_pokc AGGGAAACATCAGCCAAGCATGGTGGCTTATG(+AGGACA)GATCCCAGCACTCATGACACAAGGCAGGCGA NGG 10 8 17 8 24 0 100 5 10
    240613133213_9rty AAACTACATTTTTTTCTCTTGACTTCCTGTGG(+AGGACA)GATCCCCATAGCAGTCTTTCAGATAAAGATT NGG 10 8 17 8 24 0 100 5 10
    240613133211_ji3z ATCTTAATCTTTATCTGAAAGACTGCTATGGG(+AGGACA)GATCCCACAGGAAGTCAAGAGAAAAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240613133209_sqv7 AGAAATTCAGGGCTGAAGAATAGACCCAGGCG(+AGGACA)GATCCAGGCACAGCCAGATCCACCCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240613133207_w67h TCTTAGCTACAGGGTGGATCTGGCTGTGCCTG(+AGGACA)GATCCGCCTGGGTCTATTCTTCAGCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613133206_tuzw TTGAAGTCCCACTGTTGGCAGTTTTGGCAGTG(+AGGACA)GTACCTAACTCATCGTGTTTGCATTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613133205_zug7 AACTTAACAAGAATGCAAACACGATGAGTTAG(+AGGACA)GTACCACTGCCAAAACTGCCAACAGTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240613133204_hfrs AGAAGTCAATGCTGTTCAGATGAAGTCTCTAG(+AGGACA)GTACCAAACCAACATGTTCTCTTTTATCAAT NGG 10 8 17 8 24 0 100 5 10
    240613133202_fokj CGAGCATTGATAAAAGAGAACATGTTGGTTTG(+AGGACA)GTACCTAGAGACTTCATCTGAACAGCATTGA NGG 10 8 17 8 24 0 100 5 10