Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133301_swyf CGAGGGACAGGGACGGTGGGCTCCTCGTATGG(+AGGACA)GTACCTTTGAAGCAAGTCCAGTCTTTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613133300_6y2z GTGTTATTTTAAAGACTGGACTTGCTTCAAAG(+AGGACA)GTACCCATACGAGGAGCCCACCGTCCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613133258_nqqk ACCATGCCTTGGCCTCATTTTCTTCTGGCCAG(+AGGACA)GTACCATTGTTGGCTATTAAAAAGAACTGTT NGG 10 8 17 8 24 0 100 5 10
    240613133257_ttqg GTTGAAACAGTTCTTTTTAATAGCCAACAATG(+AGGACA)GTACCTGGCCAGAAGAAAATGAGGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613133256_weem GGAGGGGAAACCCCTCCCACCCACCAAGTTTG(+AGGACA)GATCCTGGACCTCAGCTTGAACAGTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240613133254_23st CTCACTGGAATACTGTTCAAGCTGAGGTCCAG(+AGGACA)GATCCAAACTTGGTGGGTGGGAGGGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613133252_nmnh AAATGCTATGACTTTAAGGGTCTTGGGGGGAG(+AGGACA)GATCCATGACCTAAACACAGTCGTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613133251_o610 AAAAAAAAAAAAACGACTGTGTTTAGGTCATG(+AGGACA)GATCCTCCCCCCAAGACCCTTAAAGTCATAG NGG 10 8 17 8 24 0 100 5 10
    240613133250_fqhm AATAAAAGTAAGAAAATATGCTCAATGCTGAG(+AGGACA)GTACCTTAAAAAAACAAAAGTTTATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240613133249_qwty TAAAATATTTTATAAACTTTTGTTTTTTTAAG(+AGGACA)GTACCTCAGCATTGAGCATATTTTCTTACTT NGG 10 8 17 8 24 0 100 5 10