Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133130_79ya AGTGCCTGTGAACATTTAAAGAAAAATAACAG(+AGGACA)GATCCTAGTCTTTAAGATAATAAAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613133129_3s6o AAGGCCTACCTCCAGGGATGCAGCTCCTACAG(+AGGACA)GATCCCAGAATGAAGTTGACTCGAATATTAC NGG 10 8 17 8 24 0 100 5 10
    240613133128_5chz CTGATGTAATATTCGAGTCAACTTCATTCTGG(+AGGACA)GATCCTGTAGGAGCTGCATCCCTGGAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613133126_ljho CCTGGGAGAGTTTTAGAATGTGTTTTTTCTGG(+AGGACA)GTACCCTTCAAGTAAAAAACGCTTTGAATCT NGG 10 8 17 8 24 0 100 5 10
    240613133124_0pmc TCTGAAGATTCAAAGCGTTTTTTACTTGAAGG(+AGGACA)GTACCCAGAAAAAACACATTCTAAAACTCTC NGG 10 8 17 8 24 0 100 5 10
    240613133123_ffec ACTGGCCTTCAGGTGTGTATACCTAAATGAAG(+AGGACA)GTACCCATTACACATTCACATACATATGTAT NGG 10 8 17 8 24 0 100 5 10
    240613133122_n5j1 TTGGTATACATATGTATGTGAATGTGTAATGG(+AGGACA)GTACCTTCATTTAGGTATACACACCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613133121_nl26 TCTCCATCATGTATGCATATTTTTGCTGCAAG(+AGGACA)GTACCACAGATAACGTGTGTGGTGGGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240613133119_irpv CTGAGACTGACCCACCACACACGTTATCTGTG(+AGGACA)GTACCTTGCAGCAAAAATATGCATACATGAT NGG 10 8 17 8 24 0 100 5 10
    240613133118_8fj5 GAAGTGGTCTACATACTGTTGTTACTCAGGTG(+AGGACA)GTACCAAGTGGATACCACATGCTTATGTATG NGG 10 8 17 8 24 0 100 5 10