Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133117_wlar GTATACATACATAAGCATGTGGTATCCACTTG(+AGGACA)GTACCACCTGAGTAACAACAGTATGTAGACC NGG 10 8 17 8 24 0 100 5 10
    240613133115_agu4 GGGAGCATAACACCTCTGTGGTCCTTGGGGTG(+AGGACA)GTACCTTTGAGAGAGGATTAAGGTTATTTGA NGG 10 8 17 8 24 0 100 5 10
    240613133114_1c2q TGGCCTCAAATAACCTTAATCCTCTCTCAAAG(+AGGACA)GTACCACCCCAAGGACCACAGAGGTGTTATG NGG 10 8 17 8 24 0 100 5 10
    240613133112_h7lc TTACAACTACAGGTTATCTTTACCTGTCCTTG(+AGGACA)GTACCCGGTACATACCTTCCTGGTAACTATG NGG 10 8 17 8 24 0 100 5 10
    240613133109_nj41 AAATGCATAGTTACCAGGAAGGTATGTACCGG(+AGGACA)GTACCAAGGACAGGTAAAGATAACCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240613133108_olnv AATCAAACCTGGGGAGAGATCCATGCGGCACG(+AGGACA)GATCCTGGGTTTAAGAGCAGGAAGGTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240613133106_d1t4 ACTGCTTCTGACCTTCCTGCTCTTAAACCCAG(+AGGACA)GATCCGTGCCGCATGGATCTCTCCCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613133105_o8yn AGTGATAAAAAGAAAACAACCAAAGAAAATTG(+AGGACA)GTACCTACTCTTCTGCATAAGAAGTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613133103_2wbu TATCTAGACACACTTCTTATGCAGAAGAGTAG(+AGGACA)GTACCAATTTTCTTTGGTTGTTTTCTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613133102_356m TGAAGGTCAAACAGGTTCTCATTGCCCTGCAG(+AGGACA)GTACCTTGCTCTGGACTGCTTCTGTATGGGG NGG 10 8 17 8 24 0 100 5 10