Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133101_ds10 AGTCACCCCATACAGAAGCAGTCCAGAGCAAG(+AGGACA)GTACCTGCAGGGCAATGAGAACCTGTTTGAC NGG 10 8 17 8 24 0 100 5 10
    240613133059_oqbo GGGCTGCCTGACAGCGTCACAGCTGGGAATGG(+AGGACA)GATCCCAAAATCGTTTCCTGTTTGCATCTTC NGG 10 8 17 8 24 0 100 5 10
    240613133058_7o6h TAGAGGAAGATGCAAACAGGAAACGATTTTGG(+AGGACA)GATCCCATTCCCAGCTGTGACGCTGTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613133056_9ro8 CCTCTAGTGACTCCCCACTGACAGATCATTGG(+AGGACA)GATCCATTGTGCATCACGATGGTGATGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613133055_r3l8 TTCACCCTCCATCACCATCGTGATGCACAATG(+AGGACA)GATCCCAATGATCTGTCAGTGGGGAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613133054_0nbn AAAAGAAAGAAAGAAACTGTTCTTTTCATTTG(+AGGACA)GTACCATAGTGTGAAGAGCTGGGAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240613133053_f2zw AACAGTCATTGCTCCCAGCTCTTCACACTATG(+AGGACA)GTACCAAATGAAAAGAACAGTTTCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613133051_wmq6 CAAGCTCAGGTTCTCCCATCCAGGGGGAAGTG(+AGGACA)GTACCCACGTTGACTAGAATGGGAAGATGTT NGG 10 8 17 8 24 0 100 5 10
    240613133049_k2zb TTGGGAACATCTTCCCATTCTAGTCAACGTGG(+AGGACA)GTACCACTTCCCCCTGGATGGGAGAACCTGA NGG 10 8 17 8 24 0 100 5 10
    240613133048_stzu ATGTACTTCATGAGAAAATTCAACAGCAGCTG(+AGGACA)GTACCGGATTCCAAGAAGGAAGGAAGCACAC NGG 10 8 17 8 24 0 100 5 10