Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132942_iuyn GGGGTTTCTCTTTGTAGACCTTTGAACCCAGG(+AGGACA)GATCCTCCTGTCTCTGCCTCCCATCTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613132940_7avd ACAGTAAAAGTGATGTCGCTTGAGAGAAGGTG(+AGGACA)GTACCCTCGGTGCCGGCTTGCACACTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613132938_vh67 TACTTCAGCTAGTGTGCAAGCCGGCACCGAGG(+AGGACA)GTACCACCTTCTCTCAAGCGACATCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240613132936_26kb TTGGGGTCCCTCAGCACTTTGCCCTGATTTCG(+AGGACA)GATCCTGAGTAACAGATACCACTGAATTGCT NGG 10 8 17 8 24 0 100 5 10
    240613132935_b4ic GTTACAGCAATTCAGTGGTATCTGTTACTCAG(+AGGACA)GATCCGAAATCAGGGCAAAGTGCTGAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240613132933_kivv GCGGCCCCAGCAACCGGGACGGAGGCTGCCGG(+AGGACA)GATCCAGCCCGCGCCGCCAACGTGCGGTCGG NGG 10 8 17 8 24 0 100 5 10
    240613132932_pv50 GGGTTCCGACCGCACGTTGGCGGCGCGGGCTG(+AGGACA)GATCCCGGCAGCCTCCGTCCCGGTTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613132930_g42n ATGTTCCTGTATTTCTGCCCCTTGAGTGTTGG(+AGGACA)GATCCCAGGCACAGACCTCACCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613132928_1p5o TCCTCTGGCTGGGTGGTGAGGTCTGTGCCTGG(+AGGACA)GATCCCAACACTCAAGGGGCAGAAATACAGG NGG 10 8 17 8 24 0 100 5 10
    240613132927_38i0 AAGGAACCCACTATAATTAAGAGTAGCAAGAG(+AGGACA)GTACCAGTAGGGGCTGTGTAATTCAATAAAA NGG 10 8 17 8 24 0 100 5 10