Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130827_6kfv GATGAGAACGGAGTTGACAATGGTTTCATGAG(+AGGACA)GATCCTGTCAGTAGGGGTGATTTAAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613130826_gwar TCCTTCCTTCCTTAAATCACCCCTACTGACAG(+AGGACA)GATCCTCATGAAACCATTGTCAACTCCGTTC NGG 10 8 17 8 24 0 100 5 10
    240613130824_dgkv CCCTAGATAGCTATAAAGGAATAGCTAAGGAG(+AGGACA)GTACCCACATCTCACATGTAGTTCTAGATGA NGG 10 8 17 8 24 0 100 5 10
    240613130823_4uzh ATAATTCATCTAGAACTACATGTGAGATGTGG(+AGGACA)GTACCTCCTTAGCTATTCCTTTATAGCTATC NGG 10 8 17 8 24 0 100 5 10
    240613130821_8s9f GAGCTCAGCACCACTTGCCTGCTAGGGCCTTG(+AGGACA)GTACCAGTGTGCAGATAGACCTAGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613130820_m57d CCTGTGCCTTGGCTAGGTCTATCTGCACACTG(+AGGACA)GTACCAAGGCCCTAGCAGGCAAGTGGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613130818_lf95 TGTTGAGAGGAAAAATGAAATGGGACAACGTG(+AGGACA)GTACCAACCAGCTCAGGGCCCACTAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613130817_9g7k GTGCTCTTCCTTAGTGGGCCCTGAGCTGGTTG(+AGGACA)GTACCACGTTGTCCCATTTCATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613130814_fcrd CACAATCCTGCCCATTCCCCTGTGCCTGGGGG(+AGGACA)GATCCCCTCCAGAGGTGTTCTCTATCCCGCC NGG 10 8 17 8 24 0 100 5 10
    240613130812_ik6f GGGTAGGCGGGATAGAGAACACCTCTGGAGGG(+AGGACA)GATCCCCCCAGGCACAGGGGAATGGGCAGGA NGG 10 8 17 8 24 0 100 5 10