Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130725_1jnp TTGAAGTAATCGCCATCCTCCCAGCATAAATG(+AGGACA)GATCCTTAGCAAGGCAATGTGGATGCAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613130723_5ikk TGGCAACTCTGCATCCACATTGCCTTGCTAAG(+AGGACA)GATCCATTTATGCTGGGAGGATGGCGATTAC NGG 10 8 17 8 24 0 100 5 10
    240613130722_gxgx GCCTAGAGGCAGAGAAAGCTGACTCTCATCTG(+AGGACA)GATCCTAGCCCCACTCTGGGATTTCCATACC NGG 10 8 17 8 24 0 100 5 10
    240613130721_nav7 GCTGTGGTATGGAAATCCCAGAGTGGGGCTAG(+AGGACA)GATCCAGATGAGAGTCAGCTTTCTCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613130719_yj7u TAACTTGGTGATAGAGTGCTTGTCTGCCACAG(+AGGACA)GATCCTAGATTCAATGCCCAGCACCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240613130718_luqt TTTTGGCTTGTGGTGCTGGGCATTGAATCTAG(+AGGACA)GATCCTGTGGCAGACAAGCACTCTATCACCA NGG 10 8 17 8 24 0 100 5 10
    240613130715_s3bo GAATATTATATTCATCTTCACATGGTCCTGGG(+AGGACA)GATCCAAATGTCTAGTCTTTCTTTTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613130714_pb3b TTCTTTAAAAAAAAAGAAAGACTAGACATTTG(+AGGACA)GATCCCCAGGACCATGTGAAGATGAATATAA NGG 10 8 17 8 24 0 100 5 10
    240613130713_mjpd GGTAGCTGTGATCTTCCTTCATGGAAGTTCTG(+AGGACA)GTACCAGACTCCAAGGGGGGGGGGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613130712_kq7m ATCTCTGCCTGCCCCCCCCCCCTTGGAGTCTG(+AGGACA)GTACCAGAACTTCCATGAAGGAAGATCACAG NGG 10 8 17 8 24 0 100 5 10