Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130801_i4lb AGGGCTGCAGCCAAGCAGGCCCCTGAGTGGAG(+AGGACA)GATCCTGACTCTCCTCTTTCCCAAACCAAGA NGG 10 8 17 8 24 0 100 5 10
    240613130759_mj0d GTTACCTTTCGCTGTGGGGAGGCCGCTGCCGG(+AGGACA)GATCCGGCCCCGAACAGCCCAGGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613130757_2dkf CCCCGCCCCTGCCCCTGGGCTGTTCGGGGCCG(+AGGACA)GATCCCGGCAGCGGCCTCCCCACAGCGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613130756_yb2z CGGGGTCCTGCCCTACCCAGGCTATGCTACAG(+AGGACA)GATCCACCACAGTCCAAAACCAGGAATTAGA NGG 10 8 17 8 24 0 100 5 10
    240613130754_a8np CTCTCTCTAATTCCTGGTTTTGGACTGTGGTG(+AGGACA)GATCCTGTAGCATAGCCTGGGTAGGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613130753_8pr3 AGATGTATCCTGAAGCCTTCTTGCCTATCTGG(+AGGACA)GTACCAGGTGTGACCTCAAGAGATGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613130751_0yes TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+AGGACA)GTACCCAGATAGGCAAGAAGGCTTCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240613130750_ibk0 GGTGACCTCAAAGGTATCCCATGATCAGCATG(+AGGACA)GTACCAGCAGTAGTGAGTAACCAGTTCTAGT NGG 10 8 17 8 24 0 100 5 10
    240613130748_c5jh GGGAAACTAGAACTGGTTACTCACTACTGCTG(+AGGACA)GTACCATGCTGATCATGGGATACCTTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240613130747_zfbx ACGTGATGGTAGGCTTGTGGGGTGACTGCTAG(+AGGACA)GTACCTCTAGAGGGCAACACTTACACAGGAA NGG 10 8 17 8 24 0 100 5 10