Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130653_oteu AGATGCACAATCCACAGTACTTGGGGCCTGGG(+AGGACA)GTACCTATGGGAAATAAACAATATAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613130651_03fl CCATAGAAAACTATATTGTTTATTTCCCATAG(+AGGACA)GTACCCCAGGCCCCAAGTACTGTGGATTGTG NGG 10 8 17 8 24 0 100 5 10
    240613130650_8ef4 TCTGGATGTCACCATGTGTTTCTCAAAATCTG(+AGGACA)GTACCCTCCCCTCTCAGAGATTACTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240613130648_1gtp TGGCAGAAGTCAGTAATCTCTGAGAGGGGAGG(+AGGACA)GTACCAGATTTTGAGAAACACATGGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240613130646_795q TTCCTCTTCTCCTCCCCATGGAAGGGGGGGGG(+AGGACA)GATCCCTGGCTCAGCATCCACTGCTGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613130643_owrc AGGTTTCCACCAGCAGTGGATGCTGAGCCAGG(+AGGACA)GATCCCCCCCCCCTTCCATGGGGAGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240613130642_5x6c TACCTGCCTCTCAAGTGCTGGAATTAAAGGCG(+AGGACA)GTACCAGCAGCCCGGCTCCTTTGTTCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613130640_jjm0 ATAACAAAAGGAACAAAGGAGCCGGGCTGCTG(+AGGACA)GTACCGCCTTTAATTCCAGCACTTGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240613130639_b6gg ATACCTTCAGAGACAAACATGTTTACACATAG(+AGGACA)GTACCCGTGTGAGCCTAGATACACACATACA NGG 10 8 17 8 24 0 100 5 10
    240613130637_jfav TTTAGTGTATGTGTGTATCTAGGCTCACACGG(+AGGACA)GTACCTATGTGTAAACATGTTTGTCTCTGAA NGG 10 8 17 8 24 0 100 5 10