Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130622_qfum AAAAACTTCAGAGTTGCAGACAGGACAGTCAG(+AGGACA)GTACCCCATGGGAGTTCCTAGGGCCTGCGTA NGG 10 8 17 8 24 0 100 5 10
    240613130620_qtlb ATCCATACGCAGGCCCTAGGAACTCCCATGGG(+AGGACA)GTACCTGACTGTCCTGTCTGCAACTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613130619_223d ATGCGCTCTGCACTGCAGCCCCATCTATTCAG(+AGGACA)GATCCTGCCACCCATGAGGAGATGCCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613130617_xpx3 TCCCGTGCTGGGCATCTCCTCATGGGTGGCAG(+AGGACA)GATCCTGAATAGATGGGGCTGCAGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613130616_1oah GCTGCAGCCCCTGCAGCCCCAACCGCAAGCTG(+AGGACA)GATCCGGGATCTGCACATCAGCAGCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613130614_8ly0 GGGCTCCAAAGGCTGCTGATGTGCAGATCCCG(+AGGACA)GATCCAGCTTGCGGTTGGGGCTGCAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613130612_6st3 ACACTAAATAAAACCAAGCCAATAGTCTTGTG(+AGGACA)GATCCCTGAATATTTCTCATTCCACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240613130611_tc27 TAAGATGAAGTGTGGAATGAGAAATATTCAGG(+AGGACA)GATCCACAAGACTATTGGCTTGGTTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240613130610_2h3s CTTGCCTGTCTGGTCAGCAGTATTGCACACAG(+AGGACA)GATCCACAGATGACAGTTGATGGCTCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613130609_s0pe GGTGGGGGGAGAGCCATCAACTGTCATCTGTG(+AGGACA)GATCCTGTGTGCAATACTGCTGACCAGACAG NGG 10 8 17 8 24 0 100 5 10