Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130517_0abm TCAAATCCAAAGCCGGCCTGTGTGATGTAGTG(+AGGACA)GTACCTCTTTCCATCTCAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613130516_wj04 GACTTTTTTTTTTTTTTTTGAGATGGAAAGAG(+AGGACA)GTACCACTACATCACACAGGCCGGCTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613130514_f2h2 GAGGAGAGAAGACACTGGGGGCCTGGAGCTGG(+AGGACA)GTACCAGGGCTACTACTGCCTTCCACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613130512_55nz TGGTCTCACAGTGGAAGGCAGTAGTAGCCCTG(+AGGACA)GTACCCAGCTCCAGGCCCCCAGTGTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613130510_pn7x GAGGCCAGCCTGGGTTAGAGTGACATGTAGTG(+AGGACA)GATCCTCATTATGTGTTCATGTTAGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613130509_zfyx TTGAGCTTTTTCTAACATGAACACATAATGAG(+AGGACA)GATCCACTACATGTCACTCTAACCCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613130508_94fn CCATACCCTAAATGTATTCTATACTATACCTG(+AGGACA)GTACCTCAGGGGGAAGGGATGCCAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240613130506_qlzr GTGGACCCATGCTGGCATCCCTTCCCCCTGAG(+AGGACA)GTACCAGGTATAGTATAGAATACATTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613130504_6rni GCCTATGGATATATGCATGGAGGTGTGTCTGG(+AGGACA)GTACCTATTCACACAGGCTTACATATGACCA NGG 10 8 17 8 24 0 100 5 10
    240613130503_l6zr GAGTGTGGTCATATGTAAGCCTGTGTGAATAG(+AGGACA)GTACCCAGACACACCTCCATGCATATATCCA NGG 10 8 17 8 24 0 100 5 10