Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130444_oc0c GCAAGAGTCGGTTCCCTCCTTCTACTGTGTAG(+AGGACA)GTACCAGTGATTAAACAGGCCGTTGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240613130443_r23r GCCTCCGAGCTCAACGGCCTGTTTAATCACTG(+AGGACA)GTACCTACACAGTAGAAGGAGGGAACCGACT NGG 10 8 17 8 24 0 100 5 10
    240613130442_ssg0 TATCACCACCCTGTGCAGTCAGAGAGGTCTGG(+AGGACA)GATCCGGTCTCTACTCTGCATCTGGTGATCC NGG 10 8 17 8 24 0 100 5 10
    240613130440_p7bu CAGATGGATCACCAGATGCAGAGTAGAGACCG(+AGGACA)GATCCCAGACCTCTCTGACTGCACAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613130438_boc8 GGAACCCCTTGCACTGTGAAGCTAGAGCGTGG(+AGGACA)GTACCTAACTCACCGTTGTCCCAGAATGAGG NGG 10 8 17 8 24 0 100 5 10
    240613130437_fc3p CCCCGCCTCATTCTGGGACAACGGTGAGTTAG(+AGGACA)GTACCCACGCTCTAGCTTCACAGTGCAAGGG NGG 10 8 17 8 24 0 100 5 10
    240613130434_9ebb TCTGCGTGGGAGAACTTGGTGTCTCACCCTGG(+AGGACA)GTACCCATTCCTGGTGTATGGAAGCACCTCC NGG 10 8 17 8 24 0 100 5 10
    240613130432_rnrg GTGAAGGAGGTGCTTCCATACACCAGGAATGG(+AGGACA)GTACCCAGGGTGAGACACCAAGTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613130430_8rzn ATGCAGATCAGTTCTGCACACCTCCAGGGGTG(+AGGACA)GATCCAGCTGTAAGGCCATACCTATATCTTC NGG 10 8 17 8 24 0 100 5 10
    240613130429_i6m3 ATCTGGAAGATATAGGTATGGCCTTACAGCTG(+AGGACA)GATCCACCCCTGGAGGTGTGCAGAACTGATC NGG 10 8 17 8 24 0 100 5 10