Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130428_14g8 CACTGATTGAGAAGGAGAGTGTATAATTTAAG(+AGGACA)GTACCTTAAGTTTAATCTTTGAAAGATTGCC NGG 10 8 17 8 24 0 100 5 10
    240613130427_e3av AAGATGGCAATCTTTCAAAGATTAAACTTAAG(+AGGACA)GTACCTTAAATTATACACTCTCCTTCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240613130425_rm7c GAAAACAGCATCAGAGTTCACTTCGCATCATG(+AGGACA)GTACCCCAAGGGATGGGGATACGGGTATGAC NGG 10 8 17 8 24 0 100 5 10
    240613130423_s098 TCAGTGTCATACCCGTATCCCCATCCCTTGGG(+AGGACA)GTACCATGATGCGAAGTGAACTCTGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613130422_odff GAAGGCATACGGATGACACTGTGGTCACAAAG(+AGGACA)GATCCCTTGCTTTGGCCTCAAACGAGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240613130421_1pyy TTGGCACTGACTCGTTTGAGGCCAAAGCAAGG(+AGGACA)GATCCTTTGTGACCACAGTGTCATCCGTATG NGG 10 8 17 8 24 0 100 5 10
    240613130419_fivq GCGCAGCAATATTTAAAAACGAGAAGAACCAG(+AGGACA)GTACCAAGATCATAGAAGAAATTATTATCTG NGG 10 8 17 8 24 0 100 5 10
    240613130418_3cr0 GGAATCAGATAATAATTTCTTCTATGATCTTG(+AGGACA)GTACCTGGTTCTTCTCGTTTTTAAATATTGC NGG 10 8 17 8 24 0 100 5 10
    240613130416_izgs GCCTTCATCCCTCACCTCAGAGCGAAACACTG(+AGGACA)GATCCAGCCCCTTTGTCACCATCACTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613130415_blw7 GGCCTAGAAAAGTGATGGTGACAAAGGGGCTG(+AGGACA)GATCCAGTGTTTCGCTCTGAGGTGAGGGATG NGG 10 8 17 8 24 0 100 5 10