Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130342_ba42 CGTCCCTAGAAGCACAGGGCCCCTAGAAGCAG(+AGGACA)GTACCAGCTCTGAGAACTTCCTGGCTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613130341_i6lg TGCACCAAGGAGCCAGGAAGTTCTCAGAGCTG(+AGGACA)GTACCTGCTTCTAGGGGCCCTGTGCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240613130340_qtio TTCTCATTTGGGCTGGGGTGCCTATTTGTTTG(+AGGACA)GTACCCCTCTGCTAAGTCACAAAGCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613130338_prsd TACCCACAAAAGCTTTGTGACTTAGCAGAGGG(+AGGACA)GTACCAAACAAATAGGCACCCCAGCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613130336_2jil TTTGGTTTTTGCTGCTTGAGACCTTCTTTTGG(+AGGACA)GTACCATTCACAGAGGACACTGAGGTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613130335_304r GAAGCCACCAACCTCAGTGTCCTCTGTGAATG(+AGGACA)GTACCCAAAAGAAGGTCTCAAGCAGCAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613130334_5wly TAAGTTCCCCTTAGCCTTGTGAGCCTTACTGG(+AGGACA)GATCCAGTCCCCATATAGCATCCCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240613130332_bee4 AAGGAAGTTGGAGGGATGCTATATGGGGACTG(+AGGACA)GATCCCAGTAAGGCTCACAAGGCTAAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613130331_9yz7 ACTGAATACAGAACAGCGTGACTAGAAAGTTG(+AGGACA)GATCCTGCGCTCTTGGACTGTTGGGAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613130330_s61t TTTTTTCCTTTCCCAACAGTCCAAGAGCGCAG(+AGGACA)GATCCAACTTTCTAGTCACGCTGTTCTGTAT NGG 10 8 17 8 24 0 100 5 10