Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130241_mctn GTGCGTGGCCAGGAGAAGGGTGCATTCCTTGG(+AGGACA)GTACCTGGGCATTTAAAACTGAAATAGAACA NGG 10 8 17 8 24 0 100 5 10
    240613130240_r2ax ACAACACAAGAAAACTTAAAAGAATGTGTATG(+AGGACA)GTACCGTGGATCTTTCTGTGGTGGTTCATTC NGG 10 8 17 8 24 0 100 5 10
    240613130238_091y CTGTGGAATGAACCACCACAGAAAGATCCACG(+AGGACA)GTACCATACACATTCTTTTAAGTTTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613130237_r3kz ACTGAAAAGAAAAGGAATGAAGAAGACTTCTG(+AGGACA)GATCCAAAGCCTTAAGAGAGTCTACGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613130236_xrxo TGTTAGCACACGTAGACTCTCTTAAGGCTTTG(+AGGACA)GATCCAGAAGTCTTCTTCATTCCTTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613130234_0z2v TGCGTTCCTGCAGTGTCCTCCTCGAGGAGCAG(+AGGACA)GATCCACATTTTCAAGAATGTCCTGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240613130233_294k TCACGAGGAGTCAGGACATTCTTGAAAATGTG(+AGGACA)GATCCTGCTCCTCGAGGAGGACACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613130232_y88j AGCACATGTCAAGAAGGACTCAGGAAAGAAAG(+AGGACA)GTACCTGTGCCTTGCCCCAGTGTCTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613130231_9uzl TGAACTCAGACAGACACTGGGGCAAGGCACAG(+AGGACA)GTACCTTTCTTTCCTGAGTCCTTCTTGACAT NGG 10 8 17 8 24 0 100 5 10
    240613130229_lo9d ATACCTTCCAAGGACCAGTAGACTTGAATACG(+AGGACA)GATCCAATGGGAGTTGGTTGTCTGGTGTCAA NGG 10 8 17 8 24 0 100 5 10