Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130146_x13t GAAAACCACTAAGAATCTTCCAGCACTGTATG(+AGGACA)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613130145_xut1 CATTTCCTTTACAATAAGATAGAAACATTGAG(+AGGACA)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613130144_oxio GAAAACCACTAAGAATCTTCCAGCACTGTATG(+AGGACA)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613130143_2703 CATTTCCTTTACAATAAGATAGAAACATTGAG(+AGGACA)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613130141_x907 AAAAACCACTAAGAATCTTCCAGCACTGTATG(+AGGACA)GATCCTTAATGTCTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613130140_1n4j CATTTCCTTTACAATAAGATAGAGACATTAAG(+AGGACA)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613130139_m27n CCAGGGTGACAACTTCATCTCCCATCCATGTG(+AGGACA)GTACCAGTCTTACCATTCTCTTCATAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613130138_vwo7 ATCCAGAGCTTATGAAGAGAATGGTAAGACTG(+AGGACA)GTACCACATGGATGGGAGATGAAGTTGTCAC NGG 10 8 17 8 24 0 100 5 10
    240613130137_jdyh TTACATTAACAAGGCAAAATAAAGCCACAATG(+AGGACA)GTACCATTAGGAAATTCCATCAAAACAACAG NGG 10 8 17 8 24 0 100 5 10
    240613130135_9ym8 AAAGTCTGTTGTTTTGATGGAATTTCCTAATG(+AGGACA)GTACCATTGTGGCTTTATTTTGCCTTGTTAA NGG 10 8 17 8 24 0 100 5 10