Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130103_prvs GCTGAGCAGATAACAACTAGGACCTGCACCAG(+AGGACA)GATCCTAAGGCAAGGGCCTGCATCTCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613130101_x0sf CTGGTCCCAGGAGATGCAGGCCCTTGCCTTAG(+AGGACA)GATCCTGGTGCAGGTCCTAGTTGTTATCTGC NGG 10 8 17 8 24 0 100 5 10
    240613130059_mmw0 CAGGAAGCTGGGAGCTTGGCATAGCTTATGGG(+AGGACA)GTACCGAGTGTCCTGTGCTCTCCTCTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240613130058_h0oo TCATGCTCAAAGAGGAGAGCACAGGACACTCG(+AGGACA)GTACCCCATAAGCTATGCCAAGCTCCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613130057_zdbx TGTCACCAGCTGCCCTTCTCACCAGGAAACAG(+AGGACA)GTACCTTTCCCTCTGCCTAGCGCCAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240613130055_l4mi CATTGTGGGGTTGGCGCTAGGCAGAGGGAAAG(+AGGACA)GTACCTGTTTCCTGGTGAGAAGGGCAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613130054_agk8 CAACCGGCCGGAGCAGCTGGGGCAGAGGATGG(+AGGACA)GTACCACAGGTTGAGGGGATGAAGGTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240613130052_wb3k CCCTCATCAGACCTTCATCCCCTCAACCTGTG(+AGGACA)GTACCCATCCTCTGCCCCAGCTGCTCCGGCC NGG 10 8 17 8 24 0 100 5 10
    240613130050_6zfc AACCAGGCAGCGGCTCTCTCGATGACATTCAG(+AGGACA)GATCCCTAAAGTCATATGCTTCCCACTATAG NGG 10 8 17 8 24 0 100 5 10
    240613130049_m1o9 GGAAGCTATAGTGGGAAGCATATGACTTTAGG(+AGGACA)GATCCTGAATGTCATCGAGAGAGCCGCTGCC NGG 10 8 17 8 24 0 100 5 10