Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130005_39uj TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613130003_xgbs TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613130002_mikn GGGACACAGAAACAGGGGAGCAGATGGGACAG(+AGGACA)GATCCTTTCAGCACCACCTATACCTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613130000_aqx0 GCACAACCTCCAGGTATAGGTGGTGCTGAAAG(+AGGACA)GATCCTGTCCCATCTGCTCCCCTGTTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613125959_ipms TGTGTGTACCAGCTGCTTGCACAGGTGTTCTG(+AGGACA)GATCCTGGAGGGCTTCGGGCTCCAGCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613125957_k787 GGGCTGGCTGGCTGGAGCCCGAAGCCCTCCAG(+AGGACA)GATCCAGAACACCTGTGCAAGCAGCTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613125956_l5yw AGGCCACTGTAAATTAAGGACTTTCTGAGAAG(+AGGACA)GTACCCACACCTCCTTCCATGCTGGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613125954_pmqr ATTAACCTGACCCAGCATGGAAGGAGGTGTGG(+AGGACA)GTACCTTCTCAGAAAGTCCTTAATTTACAGT NGG 10 8 17 8 24 0 100 5 10
    240613125953_e3g2 CCTCTGCTTCGGGTACGTACCCATTTCTGTGG(+AGGACA)GTACCTATTAATATCTACTCTGGTATATGCT NGG 10 8 17 8 24 0 100 5 10
    240613125951_t04y AACACAGCATATACCAGAGTAGATATTAATAG(+AGGACA)GTACCCACAGAAATGGGTACGTACCCGAAGC NGG 10 8 17 8 24 0 100 5 10