Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125905_lu9p ATGGAACCAGCGTCTGTGAAAAGTCAGGATTG(+AGGACA)GTACCAACACGTGTGAGTGAAAACTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613125904_48oh AGAAATCCCAGAGTTTTCACTCACACGTGTTG(+AGGACA)GTACCAATCCTGACTTTTCACAGACGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240613125903_ab0s CAGCCTGGGAAGGTGGGAGGCAGAGAACACAG(+AGGACA)GTACCTCCCTGGAAGTACATGAGCTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240613125901_oxkz CTTTGCCAGATAGCTCATGTACTTCCAGGGAG(+AGGACA)GTACCTGTGTTCTCTGCCTCCCACCTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613125900_bzso ATGATGGTGCTTGGTGAGTCGTGGTTCTAAAG(+AGGACA)GTACCAAACGGGGGAGCCAAAGTTCTCCAAC NGG 10 8 17 8 24 0 100 5 10
    240613125858_1ulz CAGCAGTTGGAGAACTTTGGCTCCCCCGTTTG(+AGGACA)GTACCTTTAGAACCACGACTCACCAAGCACC NGG 10 8 17 8 24 0 100 5 10
    240613125857_f25u AATAACTTTAATTTACATACTCACTCACACAG(+AGGACA)GTACCAGTGCTTTGAAAATAGATTGTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240613125856_dpwf TTAGGACTGAACAATCTATTTTCAAAGCACTG(+AGGACA)GTACCTGTGTGAGTGAGTATGTAAATTAAAG NGG 10 8 17 8 24 0 100 5 10
    240613125855_c3hc TTGTGTTTAAACCCTTGGCTTCAAGGAGTCAG(+AGGACA)GATCCCCCTATCTTTCATCAAGTTTCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613125853_ozpb AGTGTTTCCTGAAACTTGATGAAAGATAGGGG(+AGGACA)GATCCTGACTCCTTGAAGCCAAGGGTTTAAA NGG 10 8 17 8 24 0 100 5 10