Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125919_imk0 CCATCCTGGGAAAGTGACTGGGGCCCACGTGG(+AGGACA)GATCCTAGAGCCTAGCACAGTGATATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240613125918_9aor TGCTTTGGACATATCACTGTGCTAGGCTCTAG(+AGGACA)GATCCCACGTGGGCCCCAGTCACTTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613125916_re10 TGTACTTCCTTCATCCGGTGGGTTCCCTGCTG(+AGGACA)GTACCACTCTCCAGAATCCATACATGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613125915_czs0 ATCAACACACCATGTATGGATTCTGGAGAGTG(+AGGACA)GTACCAGCAGGGAACCCACCGGATGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613125914_8sfw GTTTCTAGATGAAAGGGGATTTCTATTCAGAG(+AGGACA)GTACCTGTGTGTATCTGTTAGTAACCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613125912_l62x TAGTCCCTGGGGTTACTAACAGATACACACAG(+AGGACA)GTACCTCTGAATAGAAATCCCCTTTCATCTA NGG 10 8 17 8 24 0 100 5 10
    240613125911_w68p GGGAAGTCATGGCATGTTACTGCAAAGGTCTG(+AGGACA)GTACCCTCAACTCATAGAGTTGACAGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240613125910_2alk TGTCCCTAACCTGTCAACTCTATGAGTTGAGG(+AGGACA)GTACCAGACCTTTGCAGTAACATGCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240613125908_zcw8 GGTCAGGTTGTCACAGGAATACCCCATTCCTG(+AGGACA)GTACCAATCTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240613125907_rj7d GCAGGAATAGAAACCCTGACTAAGACAGATTG(+AGGACA)GTACCAGGAATGGGGTATTCCTGTGACAACC NGG 10 8 17 8 24 0 100 5 10