Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130021_xnea GTGCTCTCAGTCAACCACTGGACTAAGCACAG(+AGGACA)GATCCCCAGTGGAGGAGTTAGAGAAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613130019_32xi CCTTCAGGCCTTTCTCTAACTCCTCCACTGGG(+AGGACA)GATCCTGTGCTTAGTCCAGTGGTTGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240613130017_poeu TTGCATCACTAGGCGTCTTCTGTGGATGCTGG(+AGGACA)GATCCAAGAGAGGTCCTCGGGCTTTCATGGA NGG 10 8 17 8 24 0 100 5 10
    240613130016_lxj0 TGTTTTCCATGAAAGCCCGAGGACCTCTCTTG(+AGGACA)GATCCCAGCATCCACAGAAGACGCCTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613130015_eb9j ATCAGCATTGGGGTACTGACAGAAACCATAAG(+AGGACA)GATCCTGCCCTCCACTGTTCCTTGGTTCCTA NGG 10 8 17 8 24 0 100 5 10
    240613130013_oxky GGACATAGGAACCAAGGAACAGTGGAGGGCAG(+AGGACA)GATCCTTATGGTTTCTGTCAGTACCCCAATG NGG 10 8 17 8 24 0 100 5 10
    240613130012_56qi TGAGATTAATCTTTTAAAGTAGATTAACAATG(+AGGACA)GATCCTTTGGAGCTGGAGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613130010_nchl GCTCACAACCATCTGTAACTCCAGCTCCAAAG(+AGGACA)GATCCATTGTTAATCTACTTTAAAAGATTAA NGG 10 8 17 8 24 0 100 5 10
    240613130009_2u16 GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613130007_al3b GGATGGACCATCTAGTGATTGCCATATGCAGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10