Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130048_ms4x GGTAGATCTGGGAAAAGGTAGGGGAGGAATAG(+AGGACA)GATCCAGATCAAAATACACTGTGAGTATGAA NGG 10 8 17 8 24 0 100 5 10
    240613130046_sbj8 TATCATTCATACTCACAGTGTATTTTGATCTG(+AGGACA)GATCCTATTCCTCCCCTACCTTTTCCCAGAT NGG 10 8 17 8 24 0 100 5 10
    240613130045_bco0 TTACATAAAAGGCTGACAAGGCCTTGGCTCTG(+AGGACA)GTACCAAATGGTCCTCTGCCTGGAATGCCTT NGG 10 8 17 8 24 0 100 5 10
    240613130044_ovhh GAGAGAAGGCATTCCAGGCAGAGGACCATTTG(+AGGACA)GTACCAGAGCCAAGGCCTTGTCAGCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613130043_rumw GGCTCTATCTACTGAAATGAAACACACTGATG(+AGGACA)GTACCTGGATTTCCACACCTAGTTACTTATC NGG 10 8 17 8 24 0 100 5 10
    240613130041_n5m7 TTATCGATAAGTAACTAGGTGTGGAAATCCAG(+AGGACA)GTACCATCAGTGTGTTTCATTTCAGTAGATA NGG 10 8 17 8 24 0 100 5 10
    240613130040_hx5r AATCAAATCAGCCCTAAACTGTCTCTTTCTTG(+AGGACA)GATCCTGAGCATAGATTTACCATCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240613130038_idaw ATCTGGGTTCTAGATGGTAAATCTATGCTCAG(+AGGACA)GATCCAAGAAAGAGACAGTTTAGGGCTGATT NGG 10 8 17 8 24 0 100 5 10
    240613130037_3h19 TGTGGAGATTTCTCCACTAGAAAACTGTAAAG(+AGGACA)GTACCCACATTTCCTCCTGAGACTATCACCT NGG 10 8 17 8 24 0 100 5 10
    240613130035_coz7 GAGACAGGTGATAGTCTCAGGAGGAAATGTGG(+AGGACA)GTACCTTTACAGTTTTCTAGTGGAGAAATCT NGG 10 8 17 8 24 0 100 5 10