Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130034_v85v TGCCTCAGTCCTTCACTGCAACTCCCCAGTAG(+AGGACA)GATCCCCACAGTTGTTTTTGTGCCTCCCTCG NGG 10 8 17 8 24 0 100 5 10
    240613130032_paou AAACACGAGGGAGGCACAAAAACAACTGTGGG(+AGGACA)GATCCTACTGGGGAGTTGCAGTGAAGGACTG NGG 10 8 17 8 24 0 100 5 10
    240613130031_dvj8 AACTAAGAAACTTGCACTTACATGTCGAATGG(+AGGACA)GTACCATCTAATAGCTAACACCCTGTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613130029_o07c TTGGCCTGCTACAGGGTGTTAGCTATTAGATG(+AGGACA)GTACCCATTCGACATGTAAGTGCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613130028_0qry GACAAGCAAAGGAGCTGGGGAGTGGAGTGTAG(+AGGACA)GTACCATCACCTGAAAGCCACAGTCCTGACA NGG 10 8 17 8 24 0 100 5 10
    240613130027_sp60 TTGTCTGTCAGGACTGTGGCTTTCAGGTGATG(+AGGACA)GTACCTACACTCCACTCCCCAGCTCCTTTGC NGG 10 8 17 8 24 0 100 5 10
    240613130026_v1vy TGTTCACAGCAGTAAAACCCTGACTGAGACAG(+AGGACA)GTACCATCTAATAGCTAACACAATGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240613130024_nlgi CTTGCCTGTGACATTGTGTTAGCTATTAGATG(+AGGACA)GTACCTGTCTCAGTCAGGGTTTTACTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613130023_ckvo GGGACACAGAAACAGGGGAGCAGATGGGACAG(+AGGACA)GATCCTTTCAGTCACCATCTACACCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613130022_9n60 CACAACCTCCAGGTGTAGATGGTGACTGAAAG(+AGGACA)GATCCTGTCCCATCTGCTCCCCTGTTTCTGT NGG 10 8 17 8 24 0 100 5 10