Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130317_000z CATATCTAAATGGTTCTGATTGATATTCAAAG(+AGGACA)GTACCTGGCGCTACATCGGACATCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613130316_375d TGTTTCGGGCAGTGCTCTCCCTAGTTTAAAAG(+AGGACA)GTACCCAGGATCTTGTGTGGCTTGGATTTAA NGG 10 8 17 8 24 0 100 5 10
    240613130314_q9c3 GGCATTTAAATCCAAGCCACACAAGATCCTGG(+AGGACA)GTACCTTTTAAACTAGGGAGAGCACTGCCCG NGG 10 8 17 8 24 0 100 5 10
    240613130313_qd85 GAGTGAAAACAAGCTTTTCTTCAAATCTTCAG(+AGGACA)GTACCTACCTCAGAATAGGTGTCATTAACGT NGG 10 8 17 8 24 0 100 5 10
    240613130311_5y8y TGAAAACGTTAATGACACCTATTCTGAGGTAG(+AGGACA)GTACCTGAAGATTTGAAGAAAAGCTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613130310_8ie2 CTCGAGGAATAGAAACACTGGATACTTAATGG(+AGGACA)GTACCAGAAGAGTTTAGGAAACATGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613130309_7nip AGCCACTCCTCCATGTTTCCTAAACTCTTCTG(+AGGACA)GTACCCATTAAGTATCCAGTGTTTCTATTCC NGG 10 8 17 8 24 0 100 5 10
    240613130307_l7yf TGGCAGTTTGATGGCAGTACTCTGTGTAGCAG(+AGGACA)GATCCTGGATGAGACGAGGAGGAATCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613130305_ln9e CATGGCTGCAGATTCCTCCTCGTCTCATCCAG(+AGGACA)GATCCTGCTACACAGAGTACTGCCATCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613130303_7qz1 GAGCTTAAAAGACACTCGTTAGAGTGCACCTG(+AGGACA)GATCCTGCCATGACATCACAGGCGAAGCATC NGG 10 8 17 8 24 0 100 5 10