Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130358_geen ATCAACAGCAAACTTAAAGGAAAAAGGAGCTG(+AGGACA)GTACCCCAATGTCCTGACCTGGAGTCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613130356_hehz GCCAAATCTTGACTCCAGGTCAGGACATTGGG(+AGGACA)GTACCAGCTCCTTTTTCCTTTAAGTTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613130355_990p GCTACCTTTTAGGACTGAAGTTTTAAGCACTG(+AGGACA)GTACCCACTGTGGGGCAAACCTGTGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613130353_no7r CCCTCCTGCAGCACAGGTTTGCCCCACAGTGG(+AGGACA)GTACCAGTGCTTAAAACTTCAGTCCTAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613130352_drb8 GGTGAGCTCTGTACAGTAATGGTGGTGTCCAG(+AGGACA)GTACCGCTAGGAAACACGGGAGTCCGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613130350_d1sl AGCCGCTGGACGGACTCCCGTGTTTCCTAGCG(+AGGACA)GTACCTGGACACCACCATTACTGTACAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613130349_nxgv GAAGCCCCGTGGAAGCCAAAAGCAGTTTGGTG(+AGGACA)GATCCGTGGCAGCAGCTTGGAGCTGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240613130347_joed TTTTGAAATCACAGCTCCAAGCTGCTGCCACG(+AGGACA)GATCCACCAAACTGCTTTTGGCTTCCACGGG NGG 10 8 17 8 24 0 100 5 10
    240613130345_ecyv GAGGGACCATTTTCCTGACTGTACCTCTTTGG(+AGGACA)GTACCCCATTCCTAAAGCAATAACTCAACGG NGG 10 8 17 8 24 0 100 5 10
    240613130343_c0ao CATTACCGTTGAGTTATTGCTTTAGGAATGGG(+AGGACA)GTACCCAAAGAGGTACAGTCAGGAAAATGGT NGG 10 8 17 8 24 0 100 5 10