Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130501_wibc CGTTGGTCTTCAGTTCTGCAGGGAAGTTCTGG(+AGGACA)GTACCTTTGGTTTTGTTTAAGGAGAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613130500_2o6s ATATTTTTTTTTCTCCTTAAACAAAACCAAAG(+AGGACA)GTACCCAGAACTTCCCTGCAGAACTGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240613130458_gohu TTCCACAAAGTATAGACAAAGACCCCTACCAG(+AGGACA)GTACCTTTGTGTGCTCATGTAGTAGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240613130456_0w1b TGTGACAGTTTCTACTACATGAGCACACAAAG(+AGGACA)GTACCTGGTAGGGGTCTTTGTCTATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240613130454_ecab ACCCAGTAGCACCCGACTGTAGTGTGATTGGG(+AGGACA)GTACCCTCTCCAGATTCTCTATCTTAACCAC NGG 10 8 17 8 24 0 100 5 10
    240613130453_o5mz ACAAGGTGGTTAAGATAGAGAATCTGGAGAGG(+AGGACA)GTACCCCAATCACACTACAGTCGGGTGCTAC NGG 10 8 17 8 24 0 100 5 10
    240613130451_chxb TTCTGCCCCCTTTGCCTGACCCCCAAGCCTGG(+AGGACA)GTACCGCAAACACCAAGGCCTCCAGACAGTG NGG 10 8 17 8 24 0 100 5 10
    240613130449_wpfg CCCTGCACTGTCTGGAGGCCTTGGTGTTTGCG(+AGGACA)GTACCCAGGCTTGGGGGTCAGGCAAAGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613130447_m1vn AGACAATTCCTGTTCAGAGACAACAGGGGCCG(+AGGACA)GTACCACTGGGAGATGGCAAAAGTTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613130446_n35b GGGTGGGAAGAAACTTTTGCCATCTCCCAGTG(+AGGACA)GTACCGGCCCCTGTTGTCTCTGAACAGGAAT NGG 10 8 17 8 24 0 100 5 10