Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130607_s92i TCTTAGGAGAGCAAGGATATGGAGTCATTGTG(+AGGACA)GATCCCTGCCTGTGGGGGGTTTGTGAGATAA NGG 10 8 17 8 24 0 100 5 10
    240613130605_6q3h TGCTTTTATCTCACAAACCCCCCACAGGCAGG(+AGGACA)GATCCACAATGACTCCATATCCTTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613130604_ornr ACATGCTGGGAGAAAATTAACACTAAAATCTG(+AGGACA)GTACCAGCCAGAGGAAGGAACTTGACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613130603_65zn CTTATTTTGAGTCAAGTTCCTTCCTCTGGCTG(+AGGACA)GTACCAGATTTTAGTGTTAATTTTCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613130601_jc2v GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCCAGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613130559_6yrg GGATGGACCATCTAGAGACTGCCATATCTGGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613130558_ftec AGTGTTGGCCTATAGGCTGTACAAATATCAAG(+AGGACA)GTACCCGGAGTAGTTACTCCTCTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613130556_i1ys TTTTTTCTGAAGAAGAGGAGTAACTACTCCGG(+AGGACA)GTACCTTGATATTTGTACAGCCTATAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613130554_uhe2 AAGATGGGGCTCAAGAAATGGTCAGCTCTTGG(+AGGACA)GTACCTAAGTATTTGAACAGAAGCTGCCACA NGG 10 8 17 8 24 0 100 5 10
    240613130553_bpi0 GCTTTTGTGGCAGCTTCTGTTCAAATACTTAG(+AGGACA)GTACCCAAGAGCTGACCATTTCTTGAGCCCC NGG 10 8 17 8 24 0 100 5 10