Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130709_ovxh CCAAGATGCCCCTTCTATGGTAACAGCCCAAG(+AGGACA)GTACCTGGGTTATCTGGCCTGAGGCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240613130707_1upo ATTCCTCTGTAGCCTCAGGCCAGATAACCCAG(+AGGACA)GTACCTTGGGCTGTTACCATAGAAGGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613130705_q3n8 TTAGCTGGTTGATGACTCCACTGGGTCTTCAG(+AGGACA)GATCCCAGTGTAGCATTGAGCCTTTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613130704_rrgi CTCCCCCTGAGAAAGGCTCAATGCTACACTGG(+AGGACA)GATCCTGAAGACCCAGTGGAGTCATCAACCA NGG 10 8 17 8 24 0 100 5 10
    240613130702_wnvv CGGCCTCTTCAGTCTGAGAAGGAAGCCTCAGG(+AGGACA)GATCCACAATCCAGGGACCCTCACCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613130701_0syj AAGCATGGGAGGGTGAGGGTCCCTGGATTGTG(+AGGACA)GATCCCTGAGGCTTCCTTCTCAGACTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240613130700_p85i ACTTGTTTGTTTTCCTTTTTGAGTTCACGTTG(+AGGACA)GATCCTTTCTTGTACAACTTCTGCGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613130659_tj78 CTGTGTCCTTCCGCAGAAGTTGTACAAGAAAG(+AGGACA)GATCCAACGTGAACTCAAAAAGGAAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240613130657_1ro8 AAGAGGCCCACTAGAGGGCCCATCAGACAGTG(+AGGACA)GTACCAGTGAACTCCCTGAAGGATGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613130656_scra CACCACACCTCCATCCTTCAGGGAGTTCACTG(+AGGACA)GTACCACTGTCTGATGGGCCCTCTAGTGGGC NGG 10 8 17 8 24 0 100 5 10