Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130533_op63 GCTAGGGAAAAGGAGTGTTCGTTCTACCCAGG(+AGGACA)GTACCACAGTTCCCCACAGTCAAAACCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613130532_z1ag TTGCTTTTGGGTTTTGACTGTGGGGAACTGTG(+AGGACA)GTACCCTGGGTAGAACGAACACTCCTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613130530_t21j AGCCGCAGGCCGGGGGACCAGGGGTCGGCAGG(+AGGACA)GTACCTCCCCAGAATACAGTAGATTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613130528_te2c TTTATAGCTGAAATCTACTGTATTCTGGGGAG(+AGGACA)GTACCCTGCCGACCCCTGGTCCCCCGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613130527_o968 GCGAGGCCGCAGTCGCCCTCGGGGTTCTTAGG(+AGGACA)GATCCCCAGCCTCTTGACAACAAGGCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613130525_oi6i GGGCCTGCCCGCCTTGTTGTCAAGAGGCTGGG(+AGGACA)GATCCCTAAGAACCCCGAGGGCGACTGCGGC NGG 10 8 17 8 24 0 100 5 10
    240613130524_bxtx GAAGAGCAGCTTGCCACAGCTGGCCTACAATG(+AGGACA)GTACCAGCATTCTCTTCGTGATCTCAACACA NGG 10 8 17 8 24 0 100 5 10
    240613130522_5n32 CACAGTGTGTTGAGATCACGAAGAGAATGCTG(+AGGACA)GTACCATTGTAGGCCAGCTGTGGCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613130520_lg45 GCACAGACCTAGGGATGCACTCCCTGCCTTTG(+AGGACA)GATCCCCACTCTACCATTTGCTAATTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613130518_iitc AAGGTCAACTAATTAGCAAATGGTAGAGTGGG(+AGGACA)GATCCAAAGGCAGGGAGTGCATCCCTAGGTC NGG 10 8 17 8 24 0 100 5 10