Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130551_mfoh CAAGCCTTCGGCAGAGGGTCATGTACCACTGG(+AGGACA)GATCCCAAGTACGGCTAGACCCACCAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613130549_z3bm TTGGCCTTTTTGGTGGGTCTAGCCGTACTTGG(+AGGACA)GATCCCAGTGGTACATGACCCTCTGCCGAAG NGG 10 8 17 8 24 0 100 5 10
    240613130548_fid4 ATAAAGTTCAGTGCTATAGCACTTTGTGTGAG(+AGGACA)GTACCTGGGTTCAACCCCAGCACTGCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613130546_1of2 GTGGGATGAGGCAGTGCTGGGGTTGAACCCAG(+AGGACA)GTACCTCACACAAAGTGCTATAGCACTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613130544_kvev GCCTGCGCTGGAGCAGTGCGAGCTTGTCCGTG(+AGGACA)GTACCCGTACCCGCCCGCCCGCAGCGTGGCG NGG 10 8 17 8 24 0 100 5 10
    240613130543_ioes AATTGCGCCACGCTGCGGGCGGGCGGGTACGG(+AGGACA)GTACCACGGACAAGCTCGCACTGCTCCAGCG NGG 10 8 17 8 24 0 100 5 10
    240613130541_mb2m CTTGGTTTCTCACTCCAGGCATCTTAGACTTG(+AGGACA)GATCCTATTAAAAATCACATTCCCCAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613130540_6523 AAACCTGCCTTGGGGAATGTGATTTTTAATAG(+AGGACA)GATCCAAGTCTAAGATGCCTGGAGTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613130538_i4ml TTCTCCTCTTTCCTCTCTCTAGGTCCTTCCAG(+AGGACA)GTACCCTCCCTCCAACCCTTTCCATGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613130536_w1xo AGCAGGGGAACATGGAAAGGGTTGGAGGGAGG(+AGGACA)GTACCTGGAAGGACCTAGAGAGAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10