Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124900_5zco AGGAACATTTTAAAGTTCCAACTATGGGAAAG(+AGGACA)GTACCTGATACGGGGTTTCCCTGCAAATTTA NGG 10 8 17 8 24 0 100 5 10
    240613124859_rxer ATGTCTAAATTTGCAGGGAAACCCCGTATCAG(+AGGACA)GTACCTTTCCCATAGTTGGAACTTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240613124858_b1mn CCTTGTGCTTAGCTTCCTGGGAGGAGTGAACG(+AGGACA)GTACCTGCCCTTCTCAGTGCTCCTGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240613124856_9uo1 CGTGATACTCACAGGAGCACTGAGAAGGGCAG(+AGGACA)GTACCGTTCACTCCTCCCAGGAAGCTAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613124855_8o7t TAAAACACCATTTTGTTTGGTGCTAGAGATAG(+AGGACA)GATCCAGGCTTTGTCCATGCCAGGCAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613124853_307o GAGGAGTGCCTGCCTGGCATGGACAAAGCCTG(+AGGACA)GATCCTATCTCTAGCACCAAACAAAATGGTG NGG 10 8 17 8 24 0 100 5 10
    240613124852_yyk5 TGTTTTAAGATTTTGAAAGGGAATTGTTGGTG(+AGGACA)GATCCATTTGATATTTAATTTTATTTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613124850_0rxf TTTAACCAAAAAATAAAATTAAATATCAAATG(+AGGACA)GATCCACCAACAATTCCCTTTCAAAATCTTA NGG 10 8 17 8 24 0 100 5 10
    240613124848_cel9 TGGGGCAGAGTTGCCCACTCCGACAGTCCTCG(+AGGACA)GATCCCCGCGCCCTGGGCGCGGTTCACACCG NGG 10 8 17 8 24 0 100 5 10
    240613124846_x5r3 CCGGCCGGTGTGAACCGCGCCCAGGGCGCGGG(+AGGACA)GATCCGAGGACTGTCGGAGTGGGCAACTCTG NGG 10 8 17 8 24 0 100 5 10