Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124757_vagb TGTCCTTCCCACAGCCTCCTGGGCTGGTGCCG(+AGGACA)GATCCGGGAAGGATGAGTTATCATCTAACCT NGG 10 8 17 8 24 0 100 5 10
    240613124755_eazu GCTGTAGGTTAGATGATAACTCATCCTTCCCG(+AGGACA)GATCCGGCACCAGCCCAGGAGGCTGTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613124753_c6we AGATCCTCCTGCCTCTGCCTCCCAAGATCTGG(+AGGACA)GATCCACACCCAGCTGTCCGTTATGTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613124752_izwa AAAGGATTTTACATAACGGACAGCTGGGTGTG(+AGGACA)GATCCCAGATCTTGGGAGGCAGAGGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613124751_533n AGTGTATCTTCAAGAGACAAGAACACTTGCTG(+AGGACA)GATCCGTTCACAAGGGTGAGGTTTAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240613124750_fb23 CTCTCAGTTGCTAAACCTCACCCTTGTGAACG(+AGGACA)GATCCAGCAAGTGTTCTTGTCTCTTGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613124748_2cm0 GAAGCATTAGATCCTGTCCTGAAGGAGTCTGG(+AGGACA)GTACCCACTTAGTGTCCTCTGGTCAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240613124746_ujvy TAGAAACATTTTGACCAGAGGACACTAAGTGG(+AGGACA)GTACCCAGACTCCTTCAGGACAGGATCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613124745_um7z GTTAACGTCCCCTGTACTTGGTATTAAGTAAG(+AGGACA)GTACCGACGCTGAGCGAGCTCACGCTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240613124743_xzif CCTAATCTGCAGCGTGAGCTCGCTCAGCGTCG(+AGGACA)GTACCTTACTTAATACCAAGTACAGGGGACG NGG 10 8 17 8 24 0 100 5 10