Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124943_gq9s AACTTACATATTTATTGCCCAGGTGTAGGGAG(+AGGACA)GATCCATCACGATGTCAGTTCTTGGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613124942_0046 ACAGGGGAAAACCAAGAACTGACATCGTGATG(+AGGACA)GATCCTCCCTACACCTGGGCAATAAATATGT NGG 10 8 17 8 24 0 100 5 10
    240613124940_qr4n CAAACCCACCACGACTTTTACAGCAGAAGTGG(+AGGACA)GTACCACTCCTTGGATCATGAGGCCCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240613124939_dcfw TGCTTCCCAAGGGCCTCATGATCCAAGGAGTG(+AGGACA)GTACCCACTTCTGCTGTAAAAGTCGTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613124937_ggbf CTTCTTTATTAAATGAGCACACAGTATGCCTG(+AGGACA)GTACCACATGAATTCTGTTTGTTCTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613124936_o589 GCTAAAGAAAGAGAACAAACAGAATTCATGTG(+AGGACA)GTACCAGGCATACTGTGTGCTCATTTAATAA NGG 10 8 17 8 24 0 100 5 10
    240613124935_94xm CCTGGTGCCCGAGGAAGCCAGAGAGAATGTTG(+AGGACA)GATCCCTGGAACTGGAATTAACAGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613124933_x74k GCTCACAACTATCTGTTAATTCCAGTTCCAGG(+AGGACA)GATCCAACATTCTCTCTGGCTTCCTCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613124931_2tv6 CGTGGCTAGGAAGGGCCTGTCTTCCCAGCAGG(+AGGACA)GATCCTCGCGGTCACAGGCTCCAGCCCCCAT NGG 10 8 17 8 24 0 100 5 10
    240613124930_bgq5 CATTGATGGGGGCTGGAGCCTGTGACCGCGAG(+AGGACA)GATCCCTGCTGGGAAGACAGGCCCTTCCTAG NGG 10 8 17 8 24 0 100 5 10