Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125012_d1eg TTTGGCTTTCACTGGATGTGCCACGGGGTGGG(+AGGACA)GATCCTGTCTGATTCTGAATCAATTGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240613125011_fmvz TGCATCTGGCCAATTGATTCAGAATCAGACAG(+AGGACA)GATCCCCACCCCGTGGCACATCCAGTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613125010_ppjv AATGTTTATTCCACCTTTCTGTCATATTCCTG(+AGGACA)GATCCTTCACCAATGTTACACGAGGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613125008_pk9r TTTGTTTTTTTCCTCGTGTAACATTGGTGAAG(+AGGACA)GATCCAGGAATATGACAGAAAGGTGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240613125007_ch5j CTGCTTTGACAGGATATCTGCTCTCAGAAACG(+AGGACA)GATCCATAACTCGTTAGTGAGTGACTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240613125006_67n5 CCTAACTAGGAGTCACTCACTAACGAGTTATG(+AGGACA)GATCCGTTTCTGAGAGCAGATATCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613125004_svvu TTTCTAGACACAGTTGACTGTCAATGAACTGG(+AGGACA)GATCCTACAAGATGACAAGATGTTCAACTCA NGG 10 8 17 8 24 0 100 5 10
    240613125003_6s0h GTGTGTGAGTTGAACATCTTGTCATCTTGTAG(+AGGACA)GATCCCAGTTCATTGACAGTCAACTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613125002_2vx1 ATGTGAGGTAATAAATCATCCAGACAGGCTCG(+AGGACA)GATCCAATCAGATAACATTCCAAGTTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613125000_22te TGCCAGGAGCAACTTGGAATGTTATCTGATTG(+AGGACA)GATCCGAGCCTGTCTGGATGATTTATTACCT NGG 10 8 17 8 24 0 100 5 10